ქიმიის ტერმინთა განმარტებითი ლექსიკონი

Immunoanalytical technique for identification of polypeptides separated using gel electrophoresis. The polypeptide is transferred by blotting to a membrane where it undergoes specific interaction with primary antibody that is specific to the protein of interest. The peptides are detected as complexes by a label and/or enzymatic reaction, which may be conjugated directly to the primary antibody or via a separate (secondary) antibody.